ppp1cb (a13528 Search Results


ppp1cb (a13528)  (ABclonal Biotechnology)
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ABclonal Biotechnology ppp1cb (a13528)
AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, <t>PPP1CB,</t> and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.
Ppp1cb (A13528), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, PPP1CB, and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.

Journal: Journal of Molecular Cell Biology

Article Title: Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA polymerase II transcription elongation

doi: 10.1093/jmcb/mjad049

Figure Lengend Snippet: AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, PPP1CB, and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.

Article Snippet: Pol II Ser2P (ab5095), Pol II Ser5P (ab5131), and PAF1 (ab20662) rabbit polyclonal antibodies were purchased from Abcam; SPT5 (A9193), SPT6 (A16434), CSTF2 (A8116), CPSF3 (A12368), SYMPK (A8722), XRN2 (A18350), PPP1CB (A13528), and PPP1CC (A4025) rabbit polyclonal antibodies were purchased from ABclonal.

Techniques: Disruption, Generated, ChIP-sequencing, Quantitative RT-PCR, Expressing, Negative Control